Tuesday, June 5, 2007

More science as art: Filtered fluorescence



This is another picture taken at work. It's three views of the same nucleus, with different filters in place for each shot. On the far left, the filter eliminates all light other than blue light. In the middle, the filter eliminates all light but red light. The picture on the right is actually a digital merge of the two pictures on the left, although filters do exist that would allow you to view both colors.

The blue staining here is the same DNA stain that I used in the "chromosomes dividing" shot. The red stain is actually an antibody that is recognizing a particular chemical modifcation in these cells. What's interesting scientifically (and also artistically) is that the regions where the blue is most intense, the red is least intense (which becomes obvious in the merge on the right).

As you can probably gather from the last two blog entries, I love fluorescence microscopy. It produces not only scientifically-compelling data but also artistically-striking images.

I'll finish this week long feature on science as art with a final image on Thursday.

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